AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transcription PCR. The use of Cyclic RT produces cDNA amplification and better results compared to conventional reverse transcription PCR – especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as reverse transcription PCR (RT-PCR). The AccuPower® CycleScript RT (dT20, dN12, or dN6) PreMix series is stable for 3 years at - 20°C due to a patented stabilizer.
AccuPower® CycleScript RT was developed for both a conventional reverse transcription PCR at a fixed temperature at 42°C as well as Cyclic Reverse Transcription that is carried out like a PCR (please see the following comparison table). Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less time compared to the conventional reverse transcription.
Conventional Reverse Transcription
|Step 1:||RNA denaturation at 65°C for 10 minutes|
|Step 2:||cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes|
Cyclic Reverse Transcription
|Step 1:||Primer annealing at a temperature between 15°C and 40°C for 30 seconds|
|Step 2:||cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes|
|Step 3 (optional):||Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature between 50°C and 55°C for 30 seconds|
Features and Benefits
|Convenient lyophilized RT premix:||Easy to use, simply add your purifies RNA|
|Broad range of working temperatures:||For RNAs that are G:C rich or significant secondary structure|
|Novel Cyclic Reverse Transcription:||Ensures that you isolate even the rarest transcripts|
|High yield of cDNA:||For genes up to 9 kb within 10 minutes|
|Stable for 2 years at -20°C:||Long life|
|RNase, DNase and Proteinase-free:||Ensures the integrity of your samples|